Part:BBa_K4807000
hemA(s) Gene - Encoding glutamyl-tRNA reductase from Salmonella arizona
hemA(s) is a mutated hemA gene from Salmonella arizona. It encodes a glutamyl-tRNA reductase and it is an upstream gene in the regulation of the metabolic flux towards 5-ALA. Reports have identified that along with hemL and hemF, upregulation of hemA(s) and hemD increases the 5-Aminolevulinic acid accumulation [1] which is a promising source for Photodynamic Therapy as a cancer treatment, because it is further metabolized into PpIX, a common PhotoSensitizer utilized in PDT techniques [2].
References
[1] Zhang, J., Kang, Z., Chen, J. et al. Optimization of the heme biosynthesis pathway for the production of 5-aminolevulinic acid in Escherichia coli. Sci Rep 5, 8584 (2015). https://doi.org/10.1038/srep08584
[2] Stummer W, Pichlmeier U, Meinel T, Wiestler OD, Zanella F, Reulen HJ: Fluorescence-guided surgery with 5-aminolevulinic acid for resection of malignant glioma: a randomised controlled multicentre phase III trial. Lancet Oncol. 2006 May;7(5):392-401
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 193
Illegal PstI site found at 950 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 193
Illegal PstI site found at 950 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 193
Illegal PstI site found at 950 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 193
Illegal PstI site found at 950 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1027
Illegal SapI.rc site found at 178
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